A practical guide to structural analysis of carbohydrates

Removal of phospholipids from LPS


  1. Check sample for the presence of C16 and C18 fatty acids by GLC.
  2. If phospholipids are present (C16 and C18 fatty acids) the extract with CHCl3:CH3OH:H2O 16:8:1
  3. Verify the absence of phospholipds by TLC (CHCl3:CH3OH:H2O:Triethylamine 30:15:2.5:0.1).