A practical guide to structural analysis of carbohydrates

6. References

Determination of the absolute configuration of mono-saccharides in complex carbohydrates by capillary G.L.C.
Gerwig GJ, Kamerling JP, Vliegenthart JF; Carbohydr Res 77 (1980) 10-17
PubMed 519653
The absolute configuration of neutral monosaccharides, 2-acetamido-2-deoxy sugars, and uronic acids can be determined by capillary g.l.c. on SE-30 after glycosidation with (-)-2-butanol and protection of the remaining polar groups. The method is illustrated by application to mixtures of the constituent sugars of the capsular polysaccharide from Klebsiella type 57, alpha 1-acid glycoprotein, mucus glycoproteins, and the methylated, acidic polysaccharide from the coccoliths of Emiliania huxleyi (Lohmann) Kamptner.

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