To determine the content of protein in biological matter one can preferably use the either the Lowry or the Bradford test (BioRad protein assay).
- Sodium deoxycholate, Na-DOC, 2% solution (2g/100mL)
- Sodium hydroxide, 4M
- Reagent D:
- Copper sulphate, 2% solution (2.5g/250mL)
- Sodium potassium tartrate, 4% solution (5g/250mL)
- Sodium carbonate, 2% solution (20g/L)
- Folin-Ciocalteaus reagent, ready-made
- 0.02% albumin solution, (20mg/100mL)
- Blank: 0.5mL water
- Take x mL protein solution and dilute to 0.5 mL. Add 0.15mL each of the Na -DOC and the NaOH solution.
- Take 0.10, 0.15 and 0.20mL of the standard albumin solution. Dilute to 0.5mL and add 0.15mL each of the NaDOC and the NaOH solution.
- Leave the samples, the standards and blank for 10 minutes.
- Make reagent D by mixing 0.5mL of CuSO4
solution, 0.5mL of Tartrate solution and 49mL of the Na2CO3
solution. Add 3.0mL of reagent D and leave for 10 minutes (maximum 3 hours)
- Folin-Ciocalteaus reagent (0.3 mL) is added immediately shaken and allowed to stand for exactly 30 min.
- Read at 750nm against blank.